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  4. Human Homocysteine(HCY)ELISA Kit

Human Homocysteine(HCY)ELISA Kit

  • 中文名稱：
    人高半胱氨酸(Hcy)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13814h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人高半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E13814h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、尿液、組織勻漿樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝過(guò)程中產(chǎn)生的中間產(chǎn)物。高Hcy水平被視為多種疾病的獨(dú)立危險(xiǎn)因素，如心血管疾病、神經(jīng)系統(tǒng)疾病等。其研究機(jī)制主要圍繞其引發(fā)血管損傷、氧化應(yīng)激、干擾細(xì)胞正常代謝等方面展開(kāi)。試劑盒檢測(cè)范圍為0.78 nmol/mL-50 nmol/mL，可廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究中Hcy代謝通路分析、疾病機(jī)制探索（如動(dòng)脈粥樣硬化、阿爾茨海默病模型研究）以及營(yíng)養(yǎng)干預(yù)或藥物開(kāi)發(fā)的療效評(píng)估，為科研人員提供高效、穩(wěn)定的Hcy濃度檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    N/A
  • 縮寫：
    Hcy
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, tissue homogenates
  • 檢測(cè)范圍：
    0.78 nmol/mL-50 nmol/mL
  • 靈敏度：
    0.195 nmol/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %98
    Range %91-105
    1:4Average %89
    Range %84-96
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-101
    EDTA plasma (n=4)9289-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/mlOD1OD2AverageCorrected
    502.082 2.156 2.119 1.997
    251.456 1.489 1.473 1.351
    12.50.941 0.968 0.955 0.833
    6.250.583 0.597 0.590 0.468
    3.1250.342 0.367 0.355 0.233
    1.560.248 0.261 0.255 0.133
    0.780.192 0.198 0.195 0.073
    00.121 0.123 0.122 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HCY antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled HCY antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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