Mouse Lipoxin A4,LXA4 ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠脂氧素A4(LXA4)酶聯(lián)免疫試劑盒（CSB-E13268m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的LXA4含量。LXA4（脂氧素A4）是一種內源性脂氧合酶衍生的類二十烷酸調節(jié)劑，具有強大的抗炎和促消炎雙重特性。其研究機制涉及抑制與ERK1/2和NF-kB通路相關的炎癥反應，并在多種生理和病理過程中發(fā)揮重要作用。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于體外實驗環(huán)境中探究炎癥反應調控機制、藥物干預對脂氧素通路的影響，或評估疾病模型中LXA4的代謝動態(tài)，為炎癥相關疾病基礎研究、藥物開發(fā)前效評價等提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

LXA4
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	98
	Range %	93-103
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of mouse LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	88-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.