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  4. Mouse Mast Cell Tryptase(MCT) ELISA Kit

Mouse Mast Cell Tryptase(MCT) ELISA Kit

  • 中文名稱：
    小鼠肥大細(xì)胞類胰蛋白酶(MCT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14326m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肥大細(xì)胞類胰蛋白酶(MCT)酶聯(lián)免疫試劑盒（CSB-E14326m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MCT含量。MCT（單羧酸轉(zhuǎn)運(yùn)體）是重要的細(xì)胞膜轉(zhuǎn)運(yùn)蛋白。背景上，它在多種細(xì)胞代謝過(guò)程中起關(guān)鍵作用。研究機(jī)制方面，主要負(fù)責(zé)轉(zhuǎn)運(yùn)單羧酸類物質(zhì)如乳酸等，在能量代謝、細(xì)胞內(nèi)pH調(diào)節(jié)等過(guò)程有重要意義，其功能異常與腫瘤等疾病發(fā)展相關(guān)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，廣泛應(yīng)用于科研領(lǐng)域，如肥大細(xì)胞活化機(jī)制研究、過(guò)敏性動(dòng)物模型評(píng)估、炎癥相關(guān)藥物篩選等場(chǎng)景，為探索MCT在免疫調(diào)控中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Tpsab1 ELISA Kit; Mcpt7 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; mMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit
  • 縮寫：
    MCT
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %89-96
    1:2Average %96
    Range %91-102
    1:4Average %97
    Range %93-100
    1:8Average %93
    Range %88-99
  • 回收率：
    The recovery of mouse MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9693-101
    EDTA plasma (n=4)9184-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.562 2.756 2.659 2.497
    10002.173 2.213 2.193 2.031
    5001.426 1.485 1.456 1.294
    2500.801 0.845 0.823 0.661
    1250.545 0.590 0.568 0.406
    62.50.309 0.293 0.301 0.139
    31.250.246 0.234 0.240 0.078
    00.168 0.155 0.162 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
  • 基因功能參考文獻(xiàn)：
    1. transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor PMID: 14527958
    2. Because mMCP-6 and mMCP-7 can compensate for each other in this mouse disease model, the elimination of both tryptases is necessary to reveal the prominent roles of these serine proteases in joint inflammation and destruction. PMID: 18668540
    3. TGF-beta stimulates mmcp-7 transcription through the Smad3-Smad4 pathway as well as c-fos induction PMID: 16730810
  • 蛋白家族：
    Peptidase S1 family, Tryptase subfamily
  • 數(shù)據(jù)庫(kù)鏈接：